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Proteintech sumo 2 3
Sumo 2 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sumo+1/SUMO2%2F3+Antibody/10__34133_slash_research__1243-286-17-19
Average 94 stars, based on 27 article reviews
sumo 2 3 - by Bioz Stars, 2026-10
94/100 stars

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Related Articles

Dot Blot:

Article Title: SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma.
Article Snippet: .. The primary antibodies, including anti-GAPDH, anti-LaminB1, anti-His, anti-HA, anti-Flag, anti-MMP2 (from proteintech), anti-Mettl3 (from abcam, Bethyl); anti-m6a (from Synaptic Systems); SUMO-1 and SUMO2/3 (from abcam); anti-MMP9, anti-E-cadherin and normal rabbit IgG (from CST); UBC9, Snail and normal mouse IgG (from santa cruz); anti-rabbit, Anti-mouse peroxidase-conjugated secondary antibodies (from proteintech). m6A dot blot assay mRNA was enriched using Dynabeads mRNA DIRECT Purification Kit (InvitrogenTM, 61012). .. After denaturation at 65°C for 5 min and chilling directly on ice, 400 ng, 200 ng, and 100 ng of samples mixed with the ice-cold 20*SSC buffer (Sigma-Aldrich) were spotted to an Amersham Hybond-N+ membrane (GE Healthcare).

Purification:

Article Title: SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma.
Article Snippet: .. The primary antibodies, including anti-GAPDH, anti-LaminB1, anti-His, anti-HA, anti-Flag, anti-MMP2 (from proteintech), anti-Mettl3 (from abcam, Bethyl); anti-m6a (from Synaptic Systems); SUMO-1 and SUMO2/3 (from abcam); anti-MMP9, anti-E-cadherin and normal rabbit IgG (from CST); UBC9, Snail and normal mouse IgG (from santa cruz); anti-rabbit, Anti-mouse peroxidase-conjugated secondary antibodies (from proteintech). m6A dot blot assay mRNA was enriched using Dynabeads mRNA DIRECT Purification Kit (InvitrogenTM, 61012). .. After denaturation at 65°C for 5 min and chilling directly on ice, 400 ng, 200 ng, and 100 ng of samples mixed with the ice-cold 20*SSC buffer (Sigma-Aldrich) were spotted to an Amersham Hybond-N+ membrane (GE Healthcare).

Membrane:

Article Title: Deciphering the endogenous SUMO-1 landscape: a novel combinatorial peptide enrichment strategy for global profiling and disease association
Article Snippet: .. In summary, after PVDF membrane incubation with a 5% skim milk block, blots were probed with antibodies against the following targets: SUMO-1 (67559-1-Ig, Proteintech) and Ulk2. ..

Incubation:

Article Title: Deciphering the endogenous SUMO-1 landscape: a novel combinatorial peptide enrichment strategy for global profiling and disease association
Article Snippet: .. In summary, after PVDF membrane incubation with a 5% skim milk block, blots were probed with antibodies against the following targets: SUMO-1 (67559-1-Ig, Proteintech) and Ulk2. ..

Article Title: ATF2-LPCAT1-Mediated PKM2 Acetylation Links Cholesterol Stress to Macrophage Metabolic Reprogramming and Functional Remodeling
Article Snippet: Pyruvate kinase M2 (PKM2) regulates cellular metabolism under stress.. However, mechanisms involving PKM2 modification in cholesterol-loaded macrophages remain unclear.. In this study, we first characterized the impact of cholesterol loading on macrophage functional and metabolic alterations.

Blocking Assay:

Article Title: Deciphering the endogenous SUMO-1 landscape: a novel combinatorial peptide enrichment strategy for global profiling and disease association
Article Snippet: .. In summary, after PVDF membrane incubation with a 5% skim milk block, blots were probed with antibodies against the following targets: SUMO-1 (67559-1-Ig, Proteintech) and Ulk2. ..

Article Title: ATF2-LPCAT1-Mediated PKM2 Acetylation Links Cholesterol Stress to Macrophage Metabolic Reprogramming and Functional Remodeling
Article Snippet: Pyruvate kinase M2 (PKM2) regulates cellular metabolism under stress.. However, mechanisms involving PKM2 modification in cholesterol-loaded macrophages remain unclear.. In this study, we first characterized the impact of cholesterol loading on macrophage functional and metabolic alterations.

Western Blot:

Article Title: Sphingosine kinase 1 aggravates liver fibrosis by mediating macrophage recruitment and polarization.
Article Snippet: .. Antibodies used for Western blotting included Arg-1 611 (rabbit, 1:1000, 16001-1-AP, Proteintech, America), ASK1 (rabbit, 1:1000, 28201-1-612 AP, Proteintech, America), JNK1/2 (rabbit, 1:1000, IPB0275, Baijia, China), KLF4 613 (rabbit, 1:1000, WL02532, Wanleibio, China), p38 (rabbit, 1:1000, WL00764, 614 Wanleibio, China), p-ASK1 (rabbit, 1:1000, 28846-1-AP, Proteintech, America), p-615 JNK1/2 (rabbit, 1:1000, BM4380, Boster, China), p-p38 (rabbit, 1:1000, #4511T, CST, 616 America), Sumo-1 (rabbit, 1:1000, 10329-1-AP, Proteintech, America), SENP1 (rabbit, 617 1:1000, 25349-1-AP, Proteintech, America), α-SMA (rabbit, 1:1000, Bs-10196R, Bioss, 618 Jo urn al Pr e-p roo f 33 China), β-actin (mouse, 1:1000, HC201, TransGen, China), GAPDH (mouse, 1:1000, 619 HC301, TransGen, China). ..

Article Title: Sphingosine Kinase 1 Aggravates Liver Fibrosis by Mediating Macrophage Recruitment and Polarization
Article Snippet: .. Antibodies used for Western blotting included Arg-1 (rabbit, 1:1000, 16001-1-AP, Proteintech), ASK1 (rabbit, 1:1000, 28201-1-AP, Proteintech), JNK1/2 (rabbit, 1:1000, IPB0275, Baijia), KLF4 (rabbit, 1:1000, WL02532, Wanleibio), p38 (rabbit, 1:1000, WL00764, Wanleibio), p-ASK1 (rabbit, 1:1000, 28846-1-AP, Proteintech), p-JNK1/2 (rabbit, 1:1000, BM4380, Boster), p-p38 (rabbit, 1:1000, 4511T, CST), Sumo-1 (rabbit, 1:1000, 10329-1-AP, Proteintech), SENP1 (rabbit, 1:1000, 25349-1-AP, Proteintech), α-SMA (rabbit, 1:1000, Bs-10196R, Bioss), β-actin (mouse, 1:1000, HC201, TransGen), GAPDH (mouse, 1:1000, HC301, TransGen). ..



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